The primer pairs we use for the PCR amplification of internal fragments of these genes are:
| Primer designation | Sequence of primers (5' - 3') | Target sequence |
PCR product length (bp) |
|---|---|---|---|
| pyr1 | GGC AAT GTC CCC CGA TGT CCT C | gdpxJ | 501 |
| pyr2 | GAT ACC CGA GAC CGA GCG AAT G | ||
| pga3 | GAT CTG GCC CTG CCA ACC ACA C | pga | 693 |
| pga4 | TTG CCC GAT CCT CCT CCA TAA A | ||
| hagB3 | CGT CGT GCA GCG GCT CCA AAA T | hagB | 560 |
| hagB4 | CGG CGC ACC ACG GCT ACC | ||
| coa1 | ACA AGG CGT GGA CAC TCA TCA AG | mcmA | 562 |
| coa2 | TGC CCG TCC TGC CCC ATT TT | ||
| pgrecA1 | TGG CCA CGG AGA AGA TAG AGA | recA | 642 |
| pgrecA2 | GAA TGT CGA TGC GGA TGG A | ||
| ftsQ1 | TTG CAG CCA ATA GTT TTC TTT TC | ftsQ | 566 |
| ftsQ2 | TTT TTC TGC CCA ATT AGG TGT A | ||
| pepO1 | ATG CCG CTG CTA AGG ACA AT | pepO | 642 |
| pepO2 | AGC TGC TTA CCA TAC CTA CG |