Genes
The Acinetobacter baumannii complex MLST scheme uses fragments of the following seven house-keeping genes:
Citrate synthase (gltA)
DNA gyrase subunit B (gyrB)
Glucose dehydrogenase B (gdhB)
Homologous recombination factor (recA)
60-kDa chaperonin (cpn60)
Glucose-6-phosphate isomerase (gpi)
RNA polymerase sigma factor(rpoD)
Primers
| Locus | Primer | Sequences | Amplicon size (bp) | Usage |
|---|---|---|---|---|
| gltA | Citrato F1 | AAT TTA CAG TGG CAC ATT AGG TCC C | 722 | amp/seq |
| Citrato R12 | GCA GAG ATA CCA GCA GAG ATA CAC G | amp/seq | ||
| gyrB | gyrB_F | TGA AGG CGG CTT ATC TGA GT | 594 | amp/seq |
| gyrB_R | GCT GGG TCT TTT TCC TGA CA | amp/seq | ||
| gdhB | GDHB 1F | GCT ACT TTT ATG CAA CAG AGC C | 774 | amp |
| GDH SEC F | ACC ACA TGC TTT GTT ATG | seq | ||
| GDHB 775R | GTT GAG TTG GCG TAT GTT GTG C | amp | ||
| GDH SEC R | GTT GGC GTA TGT TGT GC | seq | ||
| recA | RA1 | CCT GAA TCT TCY GGT AAA AC | 425 | amp/seq |
| RA2 | GTT TCT GGG CTG CCA AAC ATT AC | amp/seq | ||
| cpn60 | cpn60_F | GGT GCT CAA CTT GTT CGT GA | 640 | amp/seq |
| cpn60_R | CAC CGA AAC CAG GAG CTT TA | amp/seq | ||
| gpi | gpi_F | GAA ATT TCC GGA GCT CAC AA | 456 | amp/seq |
| gpi_R | TCA GGA GCA ATA CCC CAC TC | amp/seq | ||
| rpoD | rpoD-F | ACC CGT GAA GGT GAA ATC AG | 672 | amp/seq |
| rpoD-R | TTC AGC TGG AGC TTT AGC AAT | amp/seq |